Gene description for Cd63
Gene name CD63 antigen
Gene symbol Cd63
Other names/aliases ME491
Tspan30
Species Mus musculus
 Experiment description of studies that identified Cd63 in Melanoma cells

1
Experiment ID1167
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29907695   
OrganismMus musculus
Experiment descriptionCancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles.
AuthorsSteenbeek SC, Pham TV, de Ligt J, Zomer A, Knol JC, Piersma SR, Schelfhorst T, Huisjes R, Schiffelers RM, Cuppen E, Jimenez CR, van Rheenen J.
Journal name EMBO J.
Publication year2018
SampleMelanoma cells
Sample nameB16F1 (16.5 K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RNA sequencing
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2
Experiment ID1170
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29907695   
OrganismMus musculus
Experiment descriptionCancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles.
AuthorsSteenbeek SC, Pham TV, de Ligt J, Zomer A, Knol JC, Piersma SR, Schelfhorst T, Huisjes R, Schiffelers RM, Cuppen E, Jimenez CR, van Rheenen J.
Journal name EMBO J.
Publication year2018
SampleMelanoma cells
Sample name B16F10 (100 K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RNA sequencing
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3
Experiment ID3271
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 34593794   
OrganismMus musculus
Experiment descriptionA nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
AuthorsWang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y.
Journal name Nat Commun
Publication year2021
SampleMelanoma cells
Sample nameB16-F10
Isolation/purification methodsDifferential centrifugation
Total Exosome Isolation Reagent
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

4
Experiment ID3272
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 34593794   
OrganismMus musculus
Experiment descriptionA nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
AuthorsWang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y.
Journal name Nat Commun
Publication year2021
SampleMelanoma cells
Sample nameB16-F10
Isolation/purification methodsDifferential centrifugation
Total Exosome Isolation Reagent
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

5
Experiment ID3273
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 34593794   
OrganismMus musculus
Experiment descriptionA nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
AuthorsWang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y.
Journal name Nat Commun
Publication year2021
SampleMelanoma cells
Sample nameB16-F10
Isolation/purification methodsDifferential centrifugation
Total Exosome Isolation Reagent
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

6
Experiment ID3274
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 34593794   
OrganismMus musculus
Experiment descriptionA nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
AuthorsWang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y.
Journal name Nat Commun
Publication year2021
SampleMelanoma cells
Sample nameB16-F10
Isolation/purification methodsDifferential centrifugation
Total Exosome Isolation Reagent
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

7
Experiment ID3275
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34593794   
OrganismMus musculus
Experiment descriptionA nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
AuthorsWang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y.
Journal name Nat Commun
Publication year2021
SampleMelanoma cells
Sample nameC57BL/6 bearing B16-F10 tumour
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

8
Experiment ID3276
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34593794   
OrganismMus musculus
Experiment descriptionA nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
AuthorsWang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y.
Journal name Nat Commun
Publication year2021
SampleMelanoma cells
Sample nameC57BL/6 bearing B16-F10 tumour
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

9
Experiment ID3277
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34593794   
OrganismMus musculus
Experiment descriptionA nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
AuthorsWang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y.
Journal name Nat Commun
Publication year2021
SampleMelanoma cells
Sample nameC57BL/6 bearing B16-F10 tumour
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

10
Experiment ID3278
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34593794   
OrganismMus musculus
Experiment descriptionA nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
AuthorsWang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y.
Journal name Nat Commun
Publication year2021
SampleMelanoma cells
Sample nameC57BL/6 bearing B16-F10 tumour
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

11
Experiment ID3279
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 34593794   
OrganismMus musculus
Experiment descriptionA nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
AuthorsWang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y.
Journal name Nat Commun
Publication year2021
SampleMelanoma cells
Sample nameB16-F10
Isolation/purification methodsDifferential centrifugation
Total Exosome Isolation Reagent
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

12
Experiment ID3280
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 34593794   
OrganismMus musculus
Experiment descriptionA nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
AuthorsWang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y.
Journal name Nat Commun
Publication year2021
SampleMelanoma cells
Sample nameB16-F10
Isolation/purification methodsDifferential centrifugation
Total Exosome Isolation Reagent
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

13
Experiment ID3281
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 34593794   
OrganismMus musculus
Experiment descriptionA nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
AuthorsWang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y.
Journal name Nat Commun
Publication year2021
SampleMelanoma cells
Sample nameB16-F10
Isolation/purification methodsDifferential centrifugation
Total Exosome Isolation Reagent
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

14
Experiment ID3282
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 34593794   
OrganismMus musculus
Experiment descriptionA nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
AuthorsWang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y.
Journal name Nat Commun
Publication year2021
SampleMelanoma cells
Sample nameB16-F10
Isolation/purification methodsDifferential centrifugation
Total Exosome Isolation Reagent
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

15
Experiment ID3283
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 34593794   
OrganismMus musculus
Experiment descriptionA nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
AuthorsWang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y.
Journal name Nat Commun
Publication year2021
SampleMelanoma cells
Sample nameB16-F10
Isolation/purification methodsDifferential centrifugation
Total Exosome Isolation Reagent
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

16
Experiment ID3284
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 34593794   
OrganismMus musculus
Experiment descriptionA nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
AuthorsWang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y.
Journal name Nat Commun
Publication year2021
SampleMelanoma cells
Sample nameB16-F10
Isolation/purification methodsDifferential centrifugation
Total Exosome Isolation Reagent
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

17
Experiment ID3285
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 34593794   
OrganismMus musculus
Experiment descriptionA nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
AuthorsWang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y.
Journal name Nat Commun
Publication year2021
SampleMelanoma cells
Sample nameB16-F10
Isolation/purification methodsDifferential centrifugation
Total Exosome Isolation Reagent
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

18
Experiment ID3286
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Western blotting
PubMed ID 34593794   
OrganismMus musculus
Experiment descriptionA nanounit strategy reverses immune suppression of exosomal PD-L1 and is associated with enhanced ferroptosis
AuthorsWang G, Xie L, Li B, Sang W, Yan J, Li J, Tian H, Li W, Zhang Z, Tian Y, Dai Y.
Journal name Nat Commun
Publication year2021
SampleMelanoma cells
Sample nameB16-F10
Isolation/purification methodsDifferential centrifugation
Total Exosome Isolation Reagent
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
EV-TRACK -

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